Effects of corn resistant starch on metabolic syndrome and gut microbiota in high-fat diet-induced obese mice - 農業知識入口網

Abstract

Resistant starch, a type of dietary fiber, not only ameliorates obesity but also promotes production of short-chain fatty acid (SCFAs) and growth of beneficial
gut microbes. To determine the  bioactivity of corn resistant starch (CRS) in diet-induced obesity, mice were fed a normal diet (ND), or a high-fat diet (HFD)
with or without CRS in this study. We demonstrated that CRS  intervention mitigated HFD-induced body weight gain and adipocyte hypertrophy, 20% CRS in
particular; meanwhile, obesity-related physiological indices, including dyslipidemia, hepatic  steatosis, insulin resistance and impaired glucose tolerance, were
improved after CRS intervention. Moreover, CRS intervention alleviated the extent of inflammation in epididymal white adipose  tissues and liver tissues, as
opposed to those fed an HFD. On the other hand, CRS intervention reverted the gut microbiota composition disrupted by HFD feeding, with an increase in 
Bacteroidetes and a decrease in Firmicutes. Using 16S rRNA gene sequencing of fecal samples, we observed the relative abundances of Muribaculaceae
increased significantly after CRS  intervention, along with increased SCFA concentrations, correlated with its role in production of SCFAs; meanwhile,
endogenous probiotics Lactobacillaceae and Bifidobacteriaceae were raised,  compared to HFD feeding alone. Taken together, the bioactivity of CRS in
diet-induced obesity includes averting the progression of obesity-related metabolic disorders and reshaping the gut  microbiota composition to a state
before HFD feeding. The resulting data demonstrated the potential of CRS to be developed as a type of prebiotics and functional foods.