農業知識庫
Detection of Ralstonia solanacearum from Asymptomatic Tomato Plants, Irrigation Water, and Soil Through Non-selective Enrichment Medium with hrp Gene-Based Bio-PCR
作者：Dinesh Singh ,Shweta Sinha ,D. K. Yadav;
公佈日期：105 年 12 月 30 日
研究報告
695
Bacterial wilt of tomato caused by Ralstonia
solanacearum (Smith) Yabuuchi et al. (Microbiol Immunol
39:897–904, 1995) is a serious disease, which causes losses
up to 60 % depending on environmental conditions, soil
property, and cultivars. In present investigation, nucleotide
sequences of virulence, hypersensitive response and pathogenicity
(hrp) gene were used to design a pair of primer
(Hrp_rs 2F: 50-AGAGGTCGACGCGATACAGT-30 and
Hrp_rs 2R: 50-CATGAGCAAGGACGAAGTCA-30) for
amplification of bacterial genome. The genomic DNA of
27 isolates of R. solanacearum race 1 biovar 3 & 4 was
amplified at 323 bp. The specificity of primer was tested on
13 strains of R. solanacearum with other group of bacteria
such as Xanthomonas oryzae pv. oryzae, X. campestris pv.
campestris, and X. citri subsp. citri. Primer amplified DNA
fragment of R. solanacearum at 323 bp. The sensitivity of
the primer was 200 cfu/ml and improved further detection
level by using non-specific enrichment medium casamino
acids-pepton-glucose broth followed by PCR (BIO-PCR).
Out of 130 samples of asymptomatic tomato plants, irrigation
water, and soil collected from bacterial wilt infested
field in different agro-climatic regions of India, R. solanacearum
was detected from 86.9, 88.5, and 90.9 per cents
samples using BIO-PCR, respectively. The primer was
found specific for detecting viable and virulent strains of R.
solanacearum and useful for the diagnosis of R. solanacearum
in tomato seedlings and monitoring of pathogen in
irrigation water and soil.
知識樹分類
消費者知識庫 > 園藝類 > 觀賞植物-其他
消費者知識庫 > 其他 > 其他-其他
相關檔案下載
延伸閱讀
- 文宣－農業數位整合服務<正式上線>一支手機就搞定！115/04/01
- 文宣－115年農業經營準備金115/04/01
- 賀 本場朱堉君副研究員榮獲114年度臺灣園藝學會學術獎115/04/01
- 水果冷知識大揭密：您知道這些水果的有趣事嗎？115/02/12
- 2026全國蓮霧及棗優質管理果園評鑑 開始接受報名115/02/12
- 智慧務農 循環永續115/02/12
- 恭喜本場同仁再傳佳績！115/02/12
- 花蓮區農業專訊--水稻田及文旦園生態營造及結合企業ESG推動--03115/02/06
- 花蓮區農業專訊--水稻田及文旦園生態營造及結合企業ESG推動--02115/02/06
- 花蓮區農業專訊--水稻田及文旦園生態營造及結合企業ESG推動--01115/02/06
