農業知識庫
Multiplex PCR for simultaneous identification of Ralstonia solanacearum and Xanthomonas perforans
作者：S. Umesha;P. Avinash;
公佈日期：105 年 12 月 30 日
研究報告
915
Ralstonia solanacearum is a causative agent of
bacterial wilt in many economically important crops, and
Xanthomonas perforans is the causal organism of bacterial
spot, one of the most important diseases of vegetables. A
multiplex PCR protocol has been developed for the
simultaneous, specific and rapid identification of R. solanacearum
and X. perforans in plant materials. Speciesspecific
primers RS-F-759 and RS-R-760 for R. solanacearum,
RST2 and RST3 for X. perforans were used for
identification of both pathogens at primer concentrations of
1:4 by optimization of multiplex PCR at annealing temperature
of about 61 ± 1 C. With these primer sets, specific
amplification of 281- and 840-bp PCR products was
obtained for R. solanacearum and X. perforans, respectively.
The multiplex PCR assay was validated with susceptible
plants mechanically inoculated with both the
pathogens; specific PCR products confirmed the presence
of R. solanacearum and X. perforans. The multiplex PCR
is valuable in identification as well as primary screening of
cultivars of both pathogens. The present study is a rapid
and easy method for early identification of pathogens from
asymptomatic and symptomatic plant materials.
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